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AlphaLISA SureFire Ultra Human Phospho-IRF7 (Ser477) Detection Kit, Control Lysate

Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Human Phospho-IRF7 (Ser477) assay. This control is already provided in the AlphaLISA SureFire Ultra Human Phospho-IRF7 (Ser477) assay kit but can also be ordered separately.

Feature Specification
Application Cell Signaling
Protocol Time 2h at RT

Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Human Phospho-IRF7 (Ser477) assay. This control is already provided in the AlphaLISA SureFire Ultra Human Phospho-IRF7 (Ser477) assay kit but can also be ordered separately.

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Unit Size: 1 vial
Part #:
ALSU-PIRF7-A-L
List price
USD 159.00
Your price:
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Overview

This control lysate is intended for use with the AlphaLISA SureFire Ultra Human Phospho-IRF7 (Ser477) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, THP-1 cells were seeded at 500K cells/mL in medium containing 100 nM PMA and incubated for 24 hours. THP-1 derived macrophages were treated with 100 nM Calyculin A for 30 minutes, washed with HBSS and lysed in Lysis Buffer at a final concentration of 8 x 106 cells/mL.

Specifications

Application
Cell Signaling
Automation Compatible
Yes
Brand
AlphaLISA SureFire Ultra
Cellular or Signaling Pathway
TLR signaling
Detection Modality
Alpha
Molecular Modification
Phosphorylation
Product Group
Control Lysate
Protocol Time
2h at RT
Shipping Conditions
Shipped in Blue Ice
Target
IRF7
Target Class
Phosphoproteins
Target Species
Human
Technology
Alpha
Therapeutic Area
Inflammation
Oncology
Unit Size
1 vial

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