AlphaLISA Human Myoglobin Detection Kit, 500 Assay Points

Formats:

• Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
• Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

• No-wash steps, no separation steps
• ELISA alternative technology
• Sensitive detection
• Broad sample compatibility
• Small sample volume
• Results in less than 3 hours
• Half the time of an ELISA assay

Myoglobin is a heme protein with a molecular weight of approximately 17.5 kDa. Myoglobin is expressed in cardiac myocytes and oxidative skeletal muscle fibres. The principal function of myoglobin is to transport oxygen from the sarcolemma to the mitochondria in heart and muscle cells. Functionally, myoglobin is well accepted as an oxygen-storage protein in muscle, capable of releasing oxygen during periods of hypoxia or anoxia. After muscle tissue damage such as crush injuries, burns, myocardial infarction and muscle diseases, increased levels of myoglobin are found in the blood and urine. Myoglobin is an early marker for myocardial infarct, because it is one of the first biomarkers to rise above normal levels.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.