| Feature | Specification |
|---|---|
| Application | Protein-Protein Interaction |
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DELFIA® Eu-labeled streptavidin is intended for use as a generic reagent to detect biotin-labeled compounds, e.g. antibodies or antigens in various types of immunoassay or nucleic acid probes in hybridization assays based on solid-phase separation. The label, Eu3+, is measured using dissociation-enhanced time-resolved fluorometry.
The streptavidin is isolated from fermentation filtrates of Streptomyces avidinii and 1 mg of streptavidin binds approximately 10 μg of biotin. The preparation is supplied ready to use in 50 mmol/L Tris-HCl buffered saline solution. For DELFIA immunoassays, a final concentration of 100 ng/mL is recommended for the generation of a suitable signal.
| Application |
Protein-Protein Interaction
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
DELFIA
|
| Conjugates |
Streptavidin
|
| Detection Modality |
DELFIA
|
| Fluorophore |
Eu-N1
|
| Product Group |
Fluorescent Reagent
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target Class |
Binding Assay
|
| Technology |
TRF
|
| Unit Size |
250 µg
|
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