Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Human and Mouse High Specificity Total SMAD3 assay. This control is already provided in the AlphaLISA SureFire Ultra Human and Mouse High Specificity Total SMAD3 assay kit but can also be ordered separately.
| Feature | Specification |
|---|---|
| Application | Cell Signaling |
| Protocol Time | 2h at RT |
Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Human and Mouse High Specificity Total SMAD3 assay. This control is already provided in the AlphaLISA SureFire Ultra Human and Mouse High Specificity Total SMAD3 assay kit but can also be ordered separately.
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This control lysate is intended for use with the AlphaLISA SureFire Ultra Human and Mouse High Specificity Total SMAD3 Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, RAW 264.7 cells were cultured to confluence in T175 flasks in 10% FBS containing medium, treated with 50 ng/mL TGF-β for 90 minutes and lysed with 6 mL of Lysis Buffer.
| Application |
Cell Signaling
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra
|
| Cellular or Signaling Pathway |
Extracellular matrix remodeling / fibrosis signaling
|
| Detection Modality |
Alpha
|
| Molecular Modification |
Total
|
| Product Group |
Control Lysate
|
| Protocol Time |
2h at RT
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
SMAD3
|
| Target Class |
Phosphoproteins
|
| Target Species |
Human
Mouse
|
| Technology |
Alpha
|
| Therapeutic Area |
Inflammation
NASH/Fibrosis
Oncology
|
| Unit Size |
1 vial
|
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