LANCE Ultra Phospho-SLP76 (Ser376) Detection Kit, 500 Assay Points

Please note control lysates are NOT included in kits. Control lysates are sold separately, catalog number TRF4010S.

Formats:

• Our 500 assay point kit allows you to run 500 wells in 384-well format, using a 20 µL reaction volume.
• Our 10,000 assay point kit allows you to run 10,000 wells in 384-well format, using a 20 µL reaction volume.

LANCE^® and LANCE^® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665/615 nm X 10,000).

Lymphocyte cytosolic protein (SLP-76) is required for T-cell activation and has been implicated in a number of signaling pathways. SLP76 is phosphorylated at Ser376 by HPK1, and this phosphorylation leads to a decrease in T-cell receptor signaling.