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Automated Focus Reduction Neutralization Test Using GFP-expressing RSV

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Antibody Neutralization
Antibody Binding Inhibition
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Topic
Automated Focus Reduction Neutralization Test Using GFP-expressing RSV
Automated Focus Reduction Neutralization Test Using GFP-expressing RSV
Automated Imaging and Detection of Cytolytic Plaques
Cytomegalovirus Neutralization Assay Using Fluorescence
Zika Virus Microneutralization Assay
Cytomegalovirus Neutralization Assay Using Brightfield
  1. The host cells are seeded in a 24-well microplate and incubated for 100% confluence
  2. The cells are then infected by GFP-RSV and incubated with low, medium, and high hamster sera concentrations
  3. Finally, the plate is scanned and analyzed with the Celigo™ image cytometer Celigo Image Cytometer Celigo Image Cytometer  to count the number of foci in each well
img-automated-focus-reduction-neutralization-test-using-gfp-expressing-rsv-1.PNG

Zoomed in bright and fluorescent images of GFP-RSV infected foci

Automated focus reduction neutralization test using GFP-expressing RSV-2

Plate-view of the 24-wells showing reduction in number of GFP foci at high concentration of serum

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For research use only. Not for use in diagnostic procedures.

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