Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Human and Mouse Total Src assay. This control is already provided in the AlphaLISA SureFire Ultra Human and Mouse Total Src assay kit but can also be ordered separately.
| Feature | Specification |
|---|---|
| Application | Cell Signaling |
| Protocol Time | 2h at RT |
Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Human and Mouse Total Src assay. This control is already provided in the AlphaLISA SureFire Ultra Human and Mouse Total Src assay kit but can also be ordered separately.
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This control lysate is intended for use with the AlphaLISA SureFire Ultra Human and Mouse Total Src Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, SH-SY5Y cells were cultured to confluence in T175 flasks in 10% FBS containing media and treated with 500 μM Pervanadate for 30 minutes and lysed with 4 mL of Lysis Buffer.
| Application |
Cell Signaling
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra
|
| Cellular or Signaling Pathway |
MAPK / ERK signaling
|
| Detection Modality |
Alpha
|
| Molecular Modification |
Total
|
| Product Group |
Control Lysate
|
| Protocol Time |
2h at RT
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
Src
|
| Target Class |
Phosphoproteins
|
| Target Species |
Human
Mouse
|
| Technology |
Alpha
|
| Therapeutic Area |
Oncology
|
| Unit Size |
1 vial
|
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The definitive guide for setting up a successful AlphaLISA SureFire Ultra assay
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