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SureLight Allophycocyanin-anti-GST Antibody, 1 mg

This product has been discontinued

APC-labeled anti-GST antibody, for capturing GST-tagged proteins and peptides in LANCE TR-FRET assays.

Feature Specification
Application Protein-Protein Interaction

APC-labeled anti-GST antibody, for capturing GST-tagged proteins and peptides in LANCE TR-FRET assays.

Product variant
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).

Overview

Goat polyclonal antibody against GST conjugated to allophycocyanin (APC). APC is a fluorescent light harvesting protein unique to cyanobacteria and red algae and a member of the phycobiliprotein family of direct fluorescent dyes. Surelight APC is a chemically and intramolecularly cross-linked form of a protein. It is produced using a proprietary manufacturing process to increase thermal stability while maintaining the fluorescent and spectral properties of native dye.

LANCE® (Lanthanide chelate excite) and LANCE® Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC), etc.]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm (for ULight dye and APC).

Specifications

Application
Protein-Protein Interaction
Automation Compatible
Yes
Brand
LANCE SureLight
Conjugates
Anti-GST
Detection Modality
TR-FRET
Fluorophore
SureLight APC
Format
Microplates
Unit Size
1 mg

Resources

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Application Note Icon
Application Note
Developing a LANCE TR-FRET assay for screening PD-1 and PD-L1 binding inhibitors.

Here, we demonstrate the utility and benefits of using LANCE Ultra TR-FRET assay technology for identifying and characterizing PD...

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Technical Note
How to access true Kd values in Protein-Protein Interaction assays

Investigate the dynamics of Interactions

Kinetic values are of importance for characterizing protein-protein interactions...

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Guide
The ultimate guide to PPI assays with HTRF

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This guide will provide you with tools to help you develop your very own protein...

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