AlphaLISA Human CXCL11 Detection Kit, 500 Assay Points

Formats:

• Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
• Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

• No-wash steps, no separation steps
• ELISA alternative technology
• Sensitive detection
• Broad sample compatibility
• Small sample volume
• Results in less than 3 hours
• Half the time of an ELISA assay

Mature C-X-C Motif Chemokine 11 (CXCL11), also known as Interferon Inducible T-Cell alpha Chemoattractant (I-TAC), is a small chemokine of 73 amino acids with a molecular weight of 8.3 kDa. CXCL11 is one of the most potent chemoattractants for activated T cells and is a key player in the regulation of the immune response. It is also known to attract monocytes, B cells, Th1 lymphocytes and NK cells. CXCL11 mediates its biological actions through binding to the CXCR3 and CXCR7 receptors. CXCL11 is highly expressed in blood leukocytes, pancreas, and liver tissues and is produced, to a lower extent, by the thymus, the spleen, and the lung. Its production is induced by IFN?, and it possesses anti-microbial activity against many bacteria. CXCL11 is expressed in a variety of skin disorders, including allergic contact dermatitis, lichen planus, and mycosis fungoides.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.