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This positve control lysate is prepared from MCF7 cells, cultured to confluence in T-175 flasks in 10% FBS containing medium, then treated with 500 μg/mL Insulin for 30 minutes and lysed in 20 mL of 1X SureFire Ultra Lysis buffer.
| Application |
Cell Signaling
|
|---|---|
| Brand |
AlphaLISA SureFire Ultra
|
| Detection Modality |
Alpha
|
| Lysis Buffer Compatibility |
Lysis Buffer
|
| Molecular Modification |
Total
|
| Product Group |
Control Lysate
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
p70 S6K
|
| Target Class |
Phosphoproteins
|
| Target Species |
Human
Mouse
|
| Technology |
Alpha
|
| Therapeutic Area |
Metabolic
|
| Unit Size |
1 vial
|
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