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AlphaLISA SureFire Ultra Human Phospho-STAT4 (Tyr693) Biotin Free Detection Kit, Control Lysate

Extra control lysate for the AlphaLISA™ SureFire® Biotin-Free Human Phospho-STAT4 (Tyr693) assay. This control is already provided in the AlphaLISA SureFire® Biotin-Free Human Phospho-STAT4 (Tyr693) assay kit but can also be ordered separately.

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Feature	Specification
Application	Cell Signaling
Protocol Time	2h at RT

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Product variant

Part #:

ASBF-PST4-A-L

List price

USD 157.00

Your price:

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Product information

Overview

This control lysate is intended for use with the AlphaLISA SureFire Biotin-Free Human Phospho-STAT4 (Tyr693) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, NK-92 cells were cultured to confluence in T175 flasks with MEM-α media containing 12.5% FBS,12.5% Horse Serum, 0.2 mM inositol, 0.1 mM 2-mercaptoethanol, 0.02 mM folic acid and 200 U/mL recombinant IL-2. Cells were then deprived of IL-2 for 18 hours, treated with 200 ng/mL IL-12 for 30 minutes and lysed at 3.2 x 10⁶ cells/mL with Lysis Buffer.

Specifications

Other specifications

Application	Cell Signaling
Automation Compatible	Yes
Brand	AlphaLISA SureFire Ultra
Detection Modality	Alpha
Molecular Modification	Phosphorylation
Product Group	Control Lysate
Protocol Time	2h at RT
Shipping Conditions	Shipped in Blue Ice
Target	STAT4
Target Class	Phosphoproteins
Target Species	Human
Technology	Alpha
Therapeutic Area	Inflammation Oncology
Unit Size	1 vial