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AlphaLISA SureFire Ultra Human and Mouse Phospho-NF-κB p65 (Ser536) Biotin Free Detection Kit, Control Lysate

Extra control lysate for the AlphaLISA™ SureFire Biotin-Free Human and Mouse Phospho-NF-κB p65 (Ser536) assay. This control is already provided in the AlphaLISA SureFire Biotin-Free Human and Mouse Phospho-NF-κB p65 (Ser536) assay kit but can also be ordered separately.

Feature Specification
Application Cell Signaling
Protocol Time 2h at RT

Extra control lysate for the AlphaLISA™ SureFire Biotin-Free Human and Mouse Phospho-NF-κB p65 (Ser536) assay. This control is already provided in the AlphaLISA SureFire Biotin-Free Human and Mouse Phospho-NF-κB p65 (Ser536) assay kit but can also be ordered separately.

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Unit Size: 1 vial
Part #:
ASBF-PNFKB-A-L
List price
USD 157.00
Your price:
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Overview

This control lysate is intended for use with the AlphaLISA SureFire Biotin-Free Human and Mouse p-NFκB p65 (Ser536) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, HeLa cells were cultured to confluence in T175 flasks in 10% FBS containing media, then treated with 50 ng/mL TNFα and 20 ng/mL Calyculin A for 10 minutes and lysed with 10 mL of Lysis Buffer.

Specifications

Application
Cell Signaling
Automation Compatible
Yes
Brand
AlphaLISA SureFire Biotin-Free
Cellular or Signaling Pathway
Inflammasome/Pattern Recognition Receptors (PRRs)
Detection Modality
Alpha
Molecular Modification
Phosphorylation
Product Group
Control Lysate
Protocol Time
2h at RT
Shipping Conditions
Shipped in Blue Ice
Target
NF-κB p65
Target Class
Phosphoproteins
Target Species
Human
Mouse
Technology
Alpha
Therapeutic Area
Inflammation
Oncology
Unit Size
1 vial

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