AlphaLISA Human PTEN Detection Kit, 100 Assay Points

Formats:

• Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
• Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
• Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

• No-wash steps, no separation steps
• ELISA alternative technology
• Sensitive detection
• Broad sample compatibility
• Small sample volume

Phosphatase and tensin homolog (PTEN) is a 402 AA protein that is expressed ubiquitously in the body. The main function of PTEN is to dephosphorylate phosphatidylinositol 3,4,5-triphosphate whereby resulting in the inhibition of the AKT signaling pathway; a pathway necessary for cell survival. Through this interaction PTEN was determined to play a crucial role in tumor suppression. In cancers like prostate, endometrial, lung and breast, PTEN expression is commonly lost or highly reduced. From the established connections between PTEN and cancer, PTEN is a highly sought after cancer biomarker. The PTEN AlphaLISA detection kit allows for the detection of PTEN in tissues homogenates and cell lysates.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.