These transparent plastic cones are intended for use with Revvity’s animal anesthesia system and the 2-port rat anesthesia system manifold. They are low luminescent and low fluorescent and come in packs of four. The animal anesthesia system is designed to work with IVIS® imaging systems and allows researchers to use real-time in vivo imaging to monitor and record cellular and genetic activity within a living organism. The gas anesthesia module successfully integrates efficient gas delivery into a single system. The gas anesthesia system delivers isoflurane gas to a 5-port anesthesia manifold housed in the IVIS® system imaging chamber. The manifold safely anesthetizes up to 5 adult mice or 2 rats simultaneously. The manifold also provides waste gas scavenging ports to help prevent gas from entering the surrounding work environment. Opening the lid to the induction chamber automatically triggers a built-in vacuum system that helps ventilate waste gas away from researchers and into disposable isoflurane-absorbing charcoal filters, or into a house air exhaust system. This system allows researchers independent control and monitoring of isoflurane gas to the induction chamber and to the subjects in the imaging chamber, permitting proper dosage at all times.