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Western Lightning ONE Femto Ultra, Chemiluminescent Substrate (25 mL)

When proteins are low and antibodies are precious, our extreme-level pre-mixed one-component chemiluminescent horseradish peroxidase (HRP) substrate with low antibody consumption is the right choice. Western Lightning™ ONE Femto Ultra can detect low-femtogram protein levels in western blots with superior signal intensity.

For research use only. Not for use in diagnostic procedures.

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Offer valid until 12/15. Terms and conditions apply.

When proteins are low and antibodies are precious, our extreme-level pre-mixed one-component chemiluminescent horseradish peroxidase (HRP) substrate with low antibody consumption is the right choice. Western Lightning™ ONE Femto Ultra can detect low-femtogram protein levels in western blots with superior signal intensity.

For research use only. Not for use in diagnostic procedures.

Click to copy promo code to clipboard.
SAVE 15% NOW on online orders. Use promo code below.
YEAREND15
Offer valid until 12/15. Terms and conditions apply.
Product Variants
Unit Size: 25 mL
Part #:
NEL150001EA
List Price
USD 134.42
Unit Size: 125 mL
Part #:
NEL151001EA
List Price
USD 433.25
Unit Size: 2 x 125 mL
Part #:
NEL152001EA
List Price
USD 747.58

Overview

Our most sensitive substrate in our premier line of premixed chemiluminescent HRP substrates, Western Lightning ONE Femto Ultra offers low-femtogram detection with excellent signal intensity. Western Lightning ONE Femto Ultra is designed with maximum sensitivity in a single reagent format. Stored and ready-to-use at room temperature, the 12 month extended shelf life means you can buy and use Western Lightning ONE Femto Ultra with confidence. Available in 25 mL size.

Comparison of Western Lightning™ ONE Versus Major Competitors

Performance and Signal Duration

  • Western blotting detection of HDAC-1 in HeLa cell lysate
  • Exposure time is 120 secs for each time point
  • Images captured with ImageQuant™ LAS 4000 from GE Healthcare

Additional product information

Performance Signal Duration

Detection Level: Mid-picogram

wlo-pico-1
wlo-pico-web
  • Two-fold dilution series of HeLa whole cell lysate from 5 μg to 0.078 μg of total protein on nitrocellulose membrane
  • Primary antibody:Rabbit-anti Human HDAC-1 1:2000
  • Secondary antibody:Goat anti-rabbit IgG HRP (2mg/mL) 1:20000

Detection Level: Mid-femtogram

wlo-femto-web
wlo-femto-web
  • Two-fold dilution series of HeLa whole cell lysate from 5 μg to 0.078 μg of total protein on nitrocellulose membrane
  • Primary antibody: Rabbit-anti Human HDAC-1 1:5000
  • Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) 1:75000

Detection Level: Low-femtogram

wlo-femto-ultra-web
wlo-femto-ultra-web
  • Two-fold dilution series of HeLa whole cell lysate from 2.5 μg to 0.039 μg of total protein on nitrocellulose membrane
  • Primary antibody:  Rabbit-anti Human HDAC-1 1:10000
  • Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) 1:200000

 

Specifications

Application
Western blotting
Brand
Western Lightning ONE
Detection Modality
Chemiluminescence
Sample Type
Chemiluminescent Substrate
Shipping Conditions
Shipped Ambient
Unit Size
25 mL

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