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Western Lightning ONE Femto Ultra, Chemiluminescent Substrate (25 mL)

When proteins are low and antibodies are precious, our extreme-level pre-mixed one-component chemiluminescent horseradish peroxidase (HRP) substrate with low antibody consumption is the right choice. Western Lightning ONE Femto Ultra can detect low-femtogram protein levels in western blots with superior signal intensity.

For research use only. Not for use in diagnostic procedures.
Part Number: NEL150001EA
Unit Size: 25 mL
Part Number: NEL151001EA
Unit Size: 125 mL
Part Number: NEL152001EA
Unit Size: 2 x 125 mL
  • Our most sensitive substrate in our premier line of premixed chemiluminescent HRP substrates, Western Lightning ONE Femto Ultra offers low-femtogram detection with excellent signal intensity. Western Lightning ONE Femto Ultra is designed with maximum sensitivity in a single reagent format. Stored and ready-to-use at room temperature, the 12 month extended shelf life means you can buy and use Western Lightning ONE Femto Ultra with confidence. Available in 25 mL size.

    Comparison of Western Lightning ONE Versus Major Competitors

    Performance and Signal Duration

    • Western blotting detection of HDAC-1 in HeLa cell lysate
    • Exposure time is 120 secs for each time point
    • Images captured with ImageQuant LAS 4000 from GE Healthcare
  • Performance Signal Duration

    Detection Level: Mid-picogram

    wlo-pico-1
    wlo-pico-web
    • Two-fold dilution series of HeLa whole cell lysate from 5 μg to 0.078 μg of total protein on nitrocellulose membrane
    • Primary antibody:Rabbit-anti Human HDAC-1 1:2000
    • Secondary antibody:Goat anti-rabbit IgG HRP (2mg/mL) 1:20000

    Detection Level: Mid-femtogram

    wlo-femto-web
    wlo-femto-web
    • Two-fold dilution series of HeLa whole cell lysate from 5 μg to 0.078 μg of total protein on nitrocellulose membrane
    • Primary antibody: Rabbit-anti Human HDAC-1 1:5000
    • Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) 1:75000

    Detection Level: Low-femtogram

    wlo-femto-ultra-web
    wlo-femto-ultra-web
    • Two-fold dilution series of HeLa whole cell lysate from 2.5 μg to 0.039 μg of total protein on nitrocellulose membrane
    • Primary antibody:  Rabbit-anti Human HDAC-1 1:10000
    • Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) 1:200000

     

  • Application
    Western blotting
    Brand
    Western Lightning ONE
    Detection Method
    Chemiluminescence
    Sample Type
    Chemiluminescent Substrate
    Unit Size
    25 mL

Resources

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Flyer
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