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pHSense Mouse IgG1 Isotype Control, 50 µg

pHSense™ Mouse IgG1 Negative Control is a purified antibody designed to serve as an isotype control when working with mouse IgG1 antibodies in combination with pHSense reagents.

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Feature	Specification
Application	Internalization

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pHSense™ Mouse IgG1 Negative Control is a purified antibody designed to serve as an isotype control when working with mouse IgG1 antibodies in combination with pHSense reagents.

View product information

Product variant

Part #:

81MOIGG1NC

List price

USD 185.00

Your online price:

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Product information

Overview

pHSense Eu Fab reagents are designed to monitor antibody or anti-drug conjugate (ADC), as well as receptor- and membrane protein-mediated internalization in real time. To confidently attribute observed effects to specific antigen binding, it is crucial to include proper controls. Test antibodies may engage in non-specific interactions - particularly when their Fc regions bind to Fc receptors on cells - which can contribute to background signal. This is where negative control antibodies become essential in pHSense assays. They help distinguish true signal from background noise by revealing non-specific binding effects, thereby enhancing the reliability of your internalization measurements.

The pHSense Mouse IgG1 Negative Control is a purified mouse IgG1 antibody. It serves as an ideal isotype control when evaluating mouse IgG1 antibodies in conjunction with pHSense reagents.

Assay validation

Mouse IgG1 Negative Control application in pHSense-based internalization monitoring assay

Tag-Lite® FLAG-tagged GLP-1R cells (stable cell line, Part #: C1SU1GLP1, Revvity) were seeded in a 96-well white culture-treated plate at a density of 80,000 cells per well in complete culture medium, and incubated overnight at 37°C with 5% CO₂. A mouse IgG1 anti-FLAG antibody and the Mouse IgG1 Negative Control were pre-incubated at room temperature for 30 minutes with pHSense Eu Fab Anti-Mouse IgG1 (Part #: 81MO1EU1AA, Revvity) at a 1:2 molar ratio in cell culture medium.

Next, 40 µL of each Fab–antibody mixture were added to the cells and incubated for 1 hour at room temperature. The GLP-1R agonist Exendin-4 was then serially diluted in cell culture medium, and 10 µL of each dilution were added to the wells. After a 20-minute incubation at 37°C, the signal was recorded using a TRF-compatible plate reader.

As expected, Exendin-4 induced a dose-dependent signal increase when monitored with the anti-FLAG antibody, while no signal increase was observed with the Mouse IgG1 Negative Control.

pHSense mouse IgG1 negative control assay validation activator Internalizing antibody and ADC

Specifications

Other specifications

Application	Internalization
Brand	pHSense
Detection Modality	pH sensitive dye
Product Group	Fluorescent Reagent
Shipping Conditions	Shipped in Dry Ice
Target Class	ADCs Antibodies Cell surface proteins GPCRs
Target Species	Mouse
Technology	TRF
Unit Size	1 vial