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Phosphodiesterase [3H] cGMP SPA Enzyme Assay

cGMP radioimmunoassay kit using Scintillation Proximity Assay (SPA) beads

For research use only. Not for use in diagnostic procedures.

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cGMP radioimmunoassay kit using Scintillation Proximity Assay (SPA) beads

For research use only. Not for use in diagnostic procedures.

Click to copy promo code to clipboard.
SAVE 15% NOW on online orders. Use promo code below.
YEAREND15
Offer valid until 12/15. Terms and conditions apply.
Product Variant
Unit Size: 1 kit
Part #:
TRKQ7100
List Price
USD 5,825.00

Overview

Scintillation proximity assay (SPA) to measure cGMP-dependent PDE isoenzymes:

  • Homogeneous assay, performed in microtitre plates or tubes
  • Suitable for high throughput screening
  • Ready to use buffer
  • [3H] tracer included

    The assay concept from is based on the observation that linear nucleotides bind preferentially to SPA yttrium silicate beads compared to cyclic nucleotides in the presence of zinc sulphate. Therefore, under optimized conditions, the product of the enzyme reaction binds directly to the SPA beads, and the enzyme substrate will not. The binding of the radiolabelled product to the bead brings the isotope into close enough proximity to allow radiation from the tritium to excite the scintillant within the bead. Any unbound radiolabel is not close enough to the scintillant to allow this energy transfer, so no signal is generated. In addition, as the cyclic substrate does not bind effectively to the bead, the background signal generated is very low. A complex ion chelation mechanism enables the linear nucleotide to bind to the bead. Optimal concentrations of zinc sulphate enhance the binding and ensure robust and efficient capture. The addition of zinc sulphate in SPA bead suspension also has the inherent ability to terminate PDE reactions.

Specifications

Application
Drug Discovery & Development
Automation Compatible
No
Bead Type or Material
Yttrium Silicate (YSi)
Brand
SPA Enzymatic Assays
Detection Modality
Radiometric
Format
Microplates
Tubes
Instrument Compatibility
Microbeta2 counter
Radioisotope
3H
Shipping Conditions
Shipped in Dry Ice
Special Ordering Information
This is a radioactive product - shipping address must have a license to receive radioactive materials.
Technology
Scintilation Proximity Assay
Unit Size
1 kit

Resources

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Guide
Optimization of SPA receptor binding assays

Scintillation proximity assay has been successfully applied to receptor binding assays

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Flyer
SPA Reagents and Technologies

Combine the sensitivity of radioactive assays with the convenience of a homogeneous (no wash steps) assay for clearer research...

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