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NEXTFLEX Cell Free DNA-Seq Library Prep Kit 2.0

The NEXTFLEX®  Cell Free DNA-Seq Kit 2.0 can produce libraries from 10 ng of cell free DNA for circulating tumor DNA (ctDNA) or cell free fetal DNA (cffDNA) analysis, in two hours.

For research use only. Not for use in diagnostic procedures.
Part Number: NOVA-5150-01
Unit Size: 8 rxns
Part Number: NOVA-5150-02
Unit Size: 48 rxns
    • Designed for low sample input – 10 ng of input cfDNA required
    • Accelerated workflow with minimized hands-on time
    • Flexible adapter barcode options – up to 1,536 unique dual index barcodes are available
    • Compatible with Illumina® sequencing platforms
    • Protocols available for automation on the Sciclone® G3 NGSx, NGSx iQ and Zephyr® G3 NGS workstations

    The NEXTFLEX® Cell Free DNA-Seq Kit 2.0 delivers high coverage and reduced bias for Illumina® sequencing applications. The NEXTFLEX® Cell Free DNA-Seq Kit 2.0 can be used to prepare single, paired-end and multiplexed DNA libraries for sequencing using Illumina® platforms. The availability of up to 1,536 unique dual index barcodes facilitates high-throughput applications without compromising data integrity.

    Reduced Bias and Coverage Gaps

    NEXTFLEX® Cell Free DNA-Seq Kit 2.0 exhibits minimal GC bias and produces uniform coverage of difficult to sequence genome regions. This kit also offers robust ligation efficiency, resulting in greater library diversity and a larger number of unique sequencing reads. These optimizations enable investigators to obtain more information from each sequencing run.

    Flexible Multiplexing Options

    The NEXTFLEX® Cell Free DNA-Seq Library Prep Kit 2.0 is designed to be used with the NEXTFLEX® Unique Dual Index barcodes (384 barcodes) or the NEXTFLEX® Unique Dual Index barcodes (1,536 barcodes).

  • Brand
    NEXTFLEX
    Shipping Conditions
    Dual Temperature
    Unit Size
    8 rxns