miND Spike-In Controls

miND® Spike-In Controls are a ready-to-use mix of synthetic small RNAs added to total RNA before library prep. They have two core functions. First, they serve as a quality control that verifies the dynamic range and quantitative performance of the assay. Second, they enable absolute normalization of sequencing data, converting read counts to copies per microliter. This matters because small RNA sequencing from biofluids often varies in RNA content, making relative metrics like RPM (reads per million) potentially be misleading. By anchoring results in absolute units, miND Spike-Ins improve cross-sample and cross-batch comparability.

miND Spike-In Controls are comprised of seven oligonucleotides with unique 13 nt core sequences flanked by randomized bases, supplied in defined ratios spanning typical small-RNA abundances. They are compatible with ligation-based small RNA-seq across species and matrices, including plasma, serum, urine, CSF, synovial fluid, cells, tissues, extracellular vesicles, and non-vesicular fractions. The miND Spike-In Controls pair naturally with NEXTFLEX™ Small RNA kit as an accessory to standardize runs across studies and batches.