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Membrane Target Systems: Vasoactive Intestinal Polypeptide VIP membrane preparation

Cell membranes prepared from HT29 cells containing human endogenous Vasoactive Intestinal Polypeptide (VIP) receptor.

Some of our receptors may be restricted for sale in specified countries. Please inquire. For research use only. Not for use in diagnostic procedures.

Part number: RBHVIPM400UA
List price: USD 7,913.00
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USD 7,913.00
USD 7,913.00 /each

Overview

Membrane Target Systems are quality assured frozen membranes from cells that express Vasoactive Instestinal Peptide VIP recombinant or endogenous receptors.

We submit every batch of receptor to stringent quality control testing that includes saturation radioligand binding assay to determine receptor concentration (Bmax) and affinity (Kd). Competition binding assays are performed determine affinity (Ki) against known reference agonists and antagonists. GTPγS data is also provided for some of our Gi-coupled receptors.

Membranes are carefully prepared and ready for a variety of HTS applications, including radioligand binding (using either proximitymethods, such as FlashPlate, or classical filtration methods).

Products are packaged as frozen crude membrane preparations. One assay unit is defined as micrograms of protein, defined by competition binding assay (filtration). A complete product description and recommended protocol are included on the Product Information Sheet.

Some of our receptors may be restricted for sale in specified countries. Please inquire.

Specifications

Concentration
10 µg/µL protein
Volume
400.0 µL
Application
Drug Discovery & Development
Assay Validation
Binding
Assay Target Class
GPCR
Brand
Membrane Target Systems
Buffer
50 mM Tris-HCL (pH 7.4), 0.5mM EDTA, 10mM MgCl2, 10% sucrose
G-Alpha Natural Receptor
Gs
Host
HT29
Product Group
Membrane Preparation
Receptor Type
Vasoactive Intestinal Peptide
Species
Human
Shipping Conditions
Shipped in Dry Ice
Therapeutic Area
Cardiovascular
Central Nervous System
Inflammation
Unit Size
400 units

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