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Fluorescein-12-ddCTP
Fluorescently-labeled Fluorescein-12-ddCTP nucleotide analog for enzymatic incorporation, for direct fluorescence detection.
For research use only. Not for use in diagnostic procedures.
Product information
Overview
Fluorescein labeled dideoxycytidine triphosphate is biologically active and can be incorporated with a variety of DNA polymerases including Taq, Klenow fragment, and Sequenase™. It terminates further incorporation and enables identification of the complementary base for applications like DNA sequencing and detection of point mutations.
Specifications
| Concentration |
1 mM
|
|---|---|
| Volume |
25.0 µL
|
| Buffer |
10 mM Tris-HCl, pH 7.6, 1 mM EDTA
|
|---|---|
| Detection Method |
Direct
|
| Fluorophore |
Fluorescein
|
| Nucleotide |
ddCTP
|
| One Unit Contains |
25.0 nmol
|
| Shipping Conditions |
Shipped Ambient
|
| Unit Size |
25 nmol
|
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