Fluorescently-labeled Fluorescein-12-ddATP nucleotide analog for enzymatic incorporation, for direct fluorescence detection.
Fluorescently-labeled Fluorescein-12-ddATP nucleotide analog for enzymatic incorporation, for direct fluorescence detection.
Fluorescein labeled dideoxyadenosine triphosphate is biologically active and can be incorporated with a variety of DNA polymerases including Taq, Klenow fragment, and Sequenase™. It terminates further incorporation and enables identification of the complementary base for applications like DNA sequencing and detection of point mutations via single base extension.
Concentration |
1 mM
|
---|---|
Volume |
25.0 µL
|
Buffer/Solvent |
10 mM Tris-HCl, pH 7.6, 1 mM EDTA
|
---|---|
Detection Modality |
Direct
|
Fluorophore |
Fluorescein
|
Nucleotide |
ddATP
|
Shipping Conditions |
Shipped Ambient
|
Unit Size |
25 nmol
|
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