Fluorescently-labeled Biotin-11-ddCTP nucleotide analog for enzymatic incorporation, for direct fluorescence detection.
For research use only. Not for use in diagnostic procedures.
Biotin labeled dideoxycytosine triphosphate (ddCTP) is biologically active and can be incorporated with a variety of DNA polymerases including Taq, Klenow fragment, and Sequenase™. It terminates further incorporation and enables identification of the complementary base for applications like DNA sequencing and detection of point mutations. Biotin-labeled products may be detected with streptavidin conjugates.
The number “11” in the compound name refers to the length of the linker arm between ddCTP and the label.
Concentration |
1 mM
|
---|---|
Volume |
25.0 µL
|
Antibody Conjugates |
Biotin
|
---|---|
Buffer |
10 mM Tris-HCl, pH 7.6, 1 mM EDTA
|
Detection Method |
Indirect
|
Nucleotide |
ddCTP
|
One Unit Contains |
25.0 nmol
|
Shipping Conditions |
Shipped Ambient
|
Unit Size |
25 nmol
|
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