Biotin-11-ATP

This biotin labeled analog of adenosine triphosphate (ATP) is used for labeling RNA probes via in vitro transcription with various RNA polymerases. Biotin labeled RNA probes are used in a variety of molecular biology applications including in situ hybridization and gene expression microarrays. Biotin-labeled products may be detected with streptavidin conjugates.

The number "11" in the compound name refers to the length of the linker arm between ATP and the label.

• ATP labeling: Biotin and a fluorescent dye are attached to an ATP molecule through a flexible linker.
• Enzymatic incorporation: During in vitro transcription, RNA polymerases utilize the labeled ATP to incorporate the modified nucleotide into the elongating RNA strand, embedding the biotin and fluorescent markers directly into the probe.
• Probe detection: Once transcription is complete, the RNA probes carrying both biotin and fluorescent tags can be identified and tracked using appropriate detection methods.
• Biotin-Streptavidin binding: The biotin moiety on the RNA probe binds with high specificity and strength to streptavidin, a protein known for its exceptional affinity for biotin.
• Fluorescence detection: Streptavidin conjugated to a fluorescent dye attaches to the biotin-labeled RNA, allowing for visualization under fluorescence microscopy or other detection system.