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ProteinEXact Assay Reagent Kit

The LabChip™ ProteinEXact™ assay delivers high-resolution sizing and quantitation application for proteins ranging in size from 6.5 to 250 kDa, including bi-specific antibodies. It is well-suited for both upstream and downstream applications in biotherapeutics and gene therapy. This kit can be used to analyze up to 576 samples.
Feature Specification
Instrument Compatibility LabChip GXII Touch
Sample Type Protein
Technology Microfluidic Electrophoresis
The LabChip™ ProteinEXact™ assay delivers high-resolution sizing and quantitation application for proteins ranging in size from 6.5 to 250 kDa, including bi-specific antibodies. It is well-suited for both upstream and downstream applications in biotherapeutics and gene therapy. This kit can be used to analyze up to 576 samples.
Product variant
Unit Size: Up to 576 samples
Part #:
CLS150466
List price
USD 647.13
Your online price:
For research use only. Not for use in diagnostic procedures.

Overview

Protein Quantitation and Sizing

Bring improved analytical sensitivity to your protein quantitation and sizing applications with the ProteinEXact assay for the LabChip GXII Touch™ protein characterization system.

With the analytical power and speed of the LabChip GXII Touch protein characterization system and IntelliChip™ assay optimization technology, you can accelerate your protein development workflows with reproducible and sensitive protein quantitation, and an expanded sizing range.

  • Dependable reproducibility for quantitation (<10% CV) and sizing (<2% CV)
  • Broad sizing and quantitation range: 6.5-250 kDa and 10-2000 ng/μL
  • Sensitive: 0.2 ng/μL
  • Flexible: Run in batches of 48 or 96 samples at a time
  • Fast: Analysis in ~ 65 sec per sample

The LabChip GXII Touch protein characterization system delivers protein sizing and quantitation 46X faster run times than traditional capillary electrophoresis without compromising sensitivity, resolution or reproducibility.

The ProteinEXact Assay for sizing and quantitation applications for proteins ranging in size from 6.5 to 250 kDa.

Specifications:

  • Sizing Range: 6.5 kDa - 250 kDa
  • Sizing Precision RSD (CV): <2%
  • Sizing Resolution: ≤10%
  • Linear Dynamic Range: 10-2000 ng/µL
  • Maximum Total Protein Concentration: 2 mg/mL
  • Sensitivity Limit of Detection (LOD): 0.2 ng/µL
  • Quantitation Reproducibility: <10%
  • Sample Analysis Time: 65 sec

Additional product information

ProteinEXact Assay Reproducibility & Sensitivity
At both high and low concentration ranges, the ProteinEXact assay provides reproducibility with a CV less than 10%.


Figure 1: At both high and low concentration ranges, the ProteinEXact assay provides reproducibility with a CV less than 10%..

ProteinEXact assay is sensitive across a broad sizing range, from 6.5 to 250 kDa, starting with a concentration as low as 0.2 ng/µL.


Figure 2: ProteinEXact assay is sensitive across a broad sizing range, from 6.5 to 250 kDa, starting with a concentration as low as 0.2 ng/µL.

Specifications

Application
DNA Analysis
Automation Compatible
Yes
Instrument Compatibility
LabChip GXII Touch
Sample Type
Protein
Shipping Conditions
Dual Temperature
Technology
Microfluidic Electrophoresis
Unit Size
Up to 576 samples

Citations

  • Scheller, C., Krebs, F., Wiesner, R., Wätzig, H. and Oltmann-Norden, I. (2021), A comparative study of CE-SDS, SDS-PAGE, and Simple Western—Precision, repeatability, and apparent molecular mass shifts by glycosylation. ELECTROPHORESIS, 42: 1521-1531. https://doi.org/10.1002/elps.202100068.
  • Smith, M. T., Zhang, S., Adams, T., Dipaolo, B., & Dally, J. (2017). Establishment and validation of a microfluidic capillary gel electrophoresis platform method for purity analysis of therapeutic monoclonal antibodies. Electrophoresis,38(9-10), 1353-1365. doi:10.1002/elps.201600519.

This product is provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the data for internal research and training purposes solely in combination with Revvity, Inc. instrumentation, and not to generate revenue, which may include, but is not limited to use of the product: (1) in manufacturing or in quality assurance or quality control of a commercial product; (2) to provide a service, information, or data for a fee or other consideration; (3) for therapeutic or prophylactic purposes; (4) for diagnostic use; and (5) for resale, whether or not such items are resold for use in research. For information on purchasing a license to this product for any other purposes, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Resources

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Product Note
Automated characterization of proteins and nucleic acids

Read the LabChip® GXII Touch protein characterization system product note to learn about an automated alternative to traditional...

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Application Note
Streamlining the development of bispecific antibodies from expression to quality assessment with Revvity’s biotherapeutic workflow solutions

In this application note we show how the CHOSOURCE TnT Transposon Technology was used to express an asymmetric 4-chain bispecific...

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