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ATP Standard
ATP Standard
ATP standard primary image
Lyophilized Adenosine 5'-Triphosphate (ATP) disodium salt is specifically formulated for the generation of accurate standard curves in bioluminescence-based detection assays. Upon reconstitution with ultrapure, nuclease-free water it provides a reliable reference standard for quantifying ATP concentrations using the firefly luciferase reaction.
| Feature | Specification |
|---|---|
| Application | Cell Viability |
Lyophilized Adenosine 5'-Triphosphate (ATP) disodium salt is specifically formulated for the generation of accurate standard curves in bioluminescence-based detection assays. Upon reconstitution with ultrapure, nuclease-free water it provides a reliable reference standard for quantifying ATP concentrations using the firefly luciferase reaction.
Product variant
Unit Size: 1 vial
Part #:
6016841
List price
USD 70.00
Your online price:
For research use only. Not for use in therapeutic or diagnostic procedures.
ATP Standard
ATP standard primary image
ATP Standard
Product information
Overview
Our lyophilized ATP Standard is a reagent designed for calibrating luminescence assays with precision. Each vial undergoes testing to ensure low background interference and optimal reactivity with luciferase-luciferin reagents. The lyophilized powder offers a longer shelf-life and heat resistance compared to liquid formats. Ideal for constructing multi-point standard curves, this product enables researchers to convert luminescence units into absolute molar concentrations with confidence and reproducibility.
Preparation of 10 mM ATP Solution:
- Check the ATP amount on the vial label. (provided in μmole)
- Calculate the volume of water needed using the formula: Volume of water (μL) = ATP amount (μmole) × 100
- Example:If the label says 11.7 μmole, then: Volume = 11.7 × 100 = 1170 μL
- Add the calculated volume of water* to the vial.
- Dissolve the ATP completely by swirling the vial gently for at least one minute.
This results in a 10mM ATP solution in water.
Note: Ensure the ATP is fully dissolved before use.
*Use deionized, distilled or water for injection grade water.
Specifications
| Application |
Cell Viability
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
ATPlite
|
| Cellular or Signaling Pathway |
Autophagy
Cell Death
Cell Growth
Cytotoxicity
Necrosis
|
| Detection Modality |
Luminescence
|
| Format |
Microplates
|
| Protocol |
Standard curve
|
| Shipping Conditions |
Shipped Ambient
|
| Unit Size |
1 vial
|
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