NEXTFLEX Rapid XP V2 DNA-Seq Kit for Metagenomics

Analysis Powered by Cosmos-Hub®

The purchase of the NEXTFLEX Rapid XP v2 DNA-Seq Kit for Metagenomics includes access to Cosmos-Hub®, a secure, cloud-based analysis platform for WGS metagenomics. Cosmos-Hub® enables taxonomic and functional profiling, antimicrobial resistance (AMR) gene detection, and microbiome diversity metrics, all through version-controlled pipelines.

Key features:

• Taxonomic and functional profiling of microbial communities
• AMR gene detection and resistome summaries
• Alpha/beta diversity analysis and rich data visualizations
• Cloud access, no local infrastructure needed

If you are evaluating when to use WGS rather than amplicon approaches, start with our short blog on functional characterization of microbial communities through WGS. It explains why WGS supports both species resolution and pathway-level insights, which pairs well with KEPLER and CHAMP outputs.

KEPLER™ Host-Agnostic Microbiome Profiler

KEPLER™ Profiler delivers host-independent microbiome analysis using curated reference databases. It supports diverse sample types, without linking reads to specific hosts, making it ideal for environmental and nonclinical metagenomics.

Benefits of KEPLER™ Profiler:

• Host-agnostic profiling across sample types
• Supports taxonomic and functional output
• Over 30,000 genomes for microbial classification

Figure 1: KEPLER™ Profiler provides host-agnostic, multi-kingdom metagenomic profiling with curated reference databases, 30,000+ species coverage, strain-level placement, fast run times, patented methods, and a literature base of 600+ citations.

CHAMP™ Human Microbiome Profiler

CHAMP™ Profiler offers targeted profiling for human-associated microbiota using a validated marker gene approach. Ideal for clinical, translational, and biotech studies, CHAMP™ Profiler delivers high-resolution insights into microbial abundance, structure, and function.

Benefits of CHAMP™ Profiler:

• Enhanced detection of human-associated microbes
• Enables high-resolution taxonomic and functional profiling

Figure 2: CHAMP™ benchmark performance. CAMI shows 16% higher recall (sensitivity) vs MetaPhlAn 4. NIBSC panel shows ~400× lower false signal (~0.01%) vs other profilers.

Highly Efficient Ligation with Low Adapter Formation

The NEXTFLEX Rapid XP v2 DNA-seq Kit for Metagenomics is designed to provide exceptional ligation efficiency, which is crucial for high-quality sequencing results. The kit includes optimized reagents and protocols that ensure the efficient and accurate ligation of DNA fragments. This high ligation efficiency reduces the likelihood of adapter dimers or erroneous sequences even at low input concentrations, leading to more reliable and reproducible data. By maximizing the ligation step, the kit enhances overall workflow efficiency and contributes to the generation of high-quality sequencing libraries.

Included Normalization Beads provide highly consistent results while saving you several hours of time while preparing for sequencing

Normalization Beads offer significant benefits in DNA sequencing workflows by ensuring consistent and accurate sample preparation. They streamline the process by equalizing the concentration of DNA across different samples, which reduces variability and enhances the reliability of sequencing results. This leads to more efficient and cost-effective sequencing, as it minimizes the need for additional normalization steps and reduces the risk of errors. Overall, Normalization Beads contribute to higher quality data and more reproducible outcomes.

Flexible Multiplexing options

Barcodes are not supplied with the NEXTFLEX Rapid XP DNA-seq kit v2 for Metagenomics and need to be purchased separately. We offer 384 color-balanced NEXTFLEX UDI adapters that improve multiplexing capabilities for both low-level and high-level multiplexing needs and a set of 1,536 NEXTFLEX UDI adapters for ultra high-throughput applications.

Automation Compatibility

The NEXTFLEX Rapid XP V2 DNA-Seq kit is compatible with multiple liquid handlers, including the Sciclone G3 NGSx workstation, Sciclone G3 NGSx iQ™ workstation, and Zephyr NGS workstation

Listen to the experts.

Watch Britton Strickland, Ph.D., and Gustav Lindved in the webinar, Advanced, End-To-End Shotgun Metagenomics Workflows, as they introduce the Cosmos-Hub and how it enables the understanding of the functional roles of microbes in various environments.

Revvity Shotgun Metagenomic Sequencing Analysis - Online Demonstration

Now you can schedule a one-on-one 30-minute online demonstration that will cover everything you need to know about the Cosmos-Hub® which is now available with Revvity’s NEXTFLEX™ Rapid XP V2 DNA-Seq kit for Metagenomics. You will be able to see the analysis demonstrated and our experts will be able to answer any questions that you may have. Please choose a timeframe that suits you best and we will provide you a Microsoft Teams personal link to join when the time comes. We look forward to speaking with you.