NEXTFLEX Rapid XP v3 DNA-Seq Kit, 8 rxns

Streamlined enzymatic DNA-seq workflow

Fragmentation, end repair, and A-tailing are combined into a streamlined enzymatic reaction step, reducing workflow complexity compared to methods that require separate fragmentation and end-repair reactions.

Because fragmentation is enzymatic, library construction does not require mechanical shearing instrumentation. This simplifies setup for whole genome sequencing workflows while maintaining control over insert size and library quality.

Flexible input range with PCR and PCR-free options

The workflow supports DNA inputs from <1 ng to 500 ng. Lower-input samples can be processed with PCR amplification, while inputs ≥75 ng can be prepared using a PCR-free workflow.

PCR-free library preparation can help preserve more of the original sample complexity by avoiding amplification-associated bias. For whole genome sequencing, this can support more even genomic representation and help reduce coverage bias across the genome.

Tunable insert sizing for DNA-seq applications

Insert size can be adjusted through the enzymatic fragmentation conditions, giving users flexibility to match library construction to read length, sequencing platform, and application requirements.

Users can adjust insert size within the same enzymatic workflow, supporting method standardization across projects with different sequencing requirements.

Library quality and sequencing performance

The workflow is designed to generate high-complexity DNA-seq libraries with low adapter-dimer formation and consistent representation across a broad GC range. For whole genome sequencing, balanced coverage helps reduce the risk that sequence context, rather than sample biology, drives underrepresentation in downstream analysis.

Figure 1: Normalized WGS coverage across 20–60% GC content for eight Mimix™ reference libraries prepared using the NEXTFLEX™ Rapid XP v3 workflow. Coverage remained close to 1.0 across the evaluated GC range, indicating limited GC-associated coverage bias.

By supporting uniform coverage and efficient library construction, the Rapid XP v3 workflow helps generate sequencing-ready libraries suitable for downstream WGS analysis, including variant detection research applications.

Automation-ready library preparation

The workflow can be run manually or implemented in automated formats to scale DNA-seq library preparation across larger sample sets.

Beyond standard reaction scale, the workflow has also been evaluated under miniaturized automated conditions, generating consistent, sequencing-ready WGS libraries with reduced reagent input. For workflow setup, QC results, sequencing metrics, and variant concordance data, see the miniaturized automated library preparation application note.

Compatible NEXTFLEX accessories

The NEXTFLEX™ Rapid XP v3 DNA-Seq Kit pairs with NEXTFLEX UDI barcode adapters for sample multiplexing on Illumina® and Element Biosciences™ sequencing platforms. UDI sets, including high-throughput options up to 1,536 indexes, support scalable multiplexing across small studies, 96-well workflows, and larger sequencing programs.

For workflows that require additional post-library processing, compatible NEXTFLEX accessories may also support library normalization or downstream hybrid capture workflows.

Explore the NEXTFLEX NGS accessories portfolio to find the accessory format that best matches your workflow.