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Immunoassay platforms to fit every need
Discover our range of immunoassay technologies that enable the selection of optimal immunoassay platforms for your requirements. Choose from traditional wash-based assays to homogenous kits and toolboxes, including our AlphaLISA, HTRF, DELFIA, LANCE TR-FRET, and ELISA solutions.
Immunoassay technologies
The right technology choice for successful assays
| ELISA | DELFIA | HTRF and LANCE Ultra | AlphaLISA | |
|---|---|---|---|---|
| Browse products | ELISA products | DELFIA products | HTRF products LANCE Ultra products |
AlphaLISA products |
| Plate format | 96-wells | 96 to 384-wells | Anywhere from 96- to 1536-wells | Anywhere from 96- to 1536-wells |
| Miniaturization | Limited | Limited | Yes | Yes |
| Time to results | 4-24 hr | 4-6 hr | 1-2 hr | 1-2 hr |
| Significant time saving reallocated back to science | ||||
| Wash steps | Yes | Yes | No | No |
| While timely, in some instances wash steps can be beneficial. Wash steps may become necessary to remove non-bound analytes with higher cross-reactivity profiles and to decrease background | Absence of wash steps increases throughput, results in ease of use, reduces time to results and lowers variability | |||
| Dynamic range | 2 Log | 5 Log | 4 Log | 5 Log |
| Large dynamic ranges are beneficial to avoid making extremely large dilutions during sample preparation. Large sample dilution are cumbersome and lead to analytical imprecision/variability | ||||
| Sensitivity | 10 pg/mL | 10 pg/mL | 10 pg/mL | 1 pg/mL |
| Sufficient sensitivity to assay in serum, fluid, cell supernatant & lysates | Sufficient sensitivity to assay in cell supernatant & lysates | Sufficient sensitivity to assay in cell supernatant & lysates. May lend itself to diluting then testing serum samples, due to its superior sensitivity | ||
| Avidity | No | No | Only with stretavidin | Yes |
| Avidity may be a sought after characteristic when detecting protein-protein interactions of low affinities | ||||
| Signal stability | Up to 1 hr | Days - Years | 24 hrs to days | 24 hrs to days |
| Allows for the plates to be left on the bench and read at a convenient time. Maximizes assay precision when incubation times vary | ||||
| Multiplexing | No | Yes (up to 3 targets) | No | Yes (up to 3 targets) |
| Reader requirements | Chromogenic and Chemiluminescence Compatible | TRF Compatible | TR-FRET Compatible | Alpha Compatible |
| 100+ compatible reader | 50+ compatible reader | 10+ compatible reader | ||
| Chemistry | Enzyme-linked, HRP or AP | Fluorophore Chelate | Fluorophore Chelate (LANCE Ultra) and Fluorophore Cryptate (HTRF) | Fluorophore Embedded Beads |
| Extremely robust chemistry, light & temperature insensitive | Extremely robust chemistry, light, temperature. Ion insensitive (HTRF only) | |||
| Analyte size | No limitation | No limitation | Subject to forster radius (9 nm) | Subject to singlet oxygen diffusion (200 nm) |
| Although LANCE, HTRF and Alpha are subject to forster radius and singlet oxygen diffusion laws, these requirements are rarely a limitation to the development of a successful assay | ||||
| Browse products | ELISA products | DELFIA products | HTRF products LANCE Ultra products |
AlphaLISA products |