Media selection
Serum-free cell cultures media has undergone continuous development and optimization efforts with the goal of increasing cell density, viability, and recombinant protein titer [Ritacco et al. 2018]
Fine tuning the media for your specific application can require testing different types and amounts of metabolites, nutrient amino acids, and antioxidants (e.g. DMSO). Considering the need for different concentrations and triplicate samples, media selection can quickly become an overwhelming task that is difficult to track. Image-based cytometry can monitor proliferation and viability to simplify media selection.
The example above shows an experiment where three factors were used in different combinations (left panel). CHO cells (10/well) were plated on a 384-well plate (35 replicates per condition). On day 4, proliferation was measured with the Celigo™ image cytometer, and the highest cell counts were observed for samples grown in media containing Factor C (right panel: 1, 5, 6, 7).
Revvity can rapidly and reliably determine the cell concentrations and viabilities in the multiple plates needed to perform these optimization experiments.
- Perform label-free cell proliferation assays in 96- and 384-well plates at 5 minutes per plate
- Direct cell counting to determine expression levels from 6- to 1536-well plates
- High speed, high-throughput, whole-well imaging
High-throughput cell counter provides precise cell counting and viability for 24 samples in <3 minutes.
For research use only. Not for use in diagnostic procedures.