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Cell Counting and Image Cytometry

Automated Imaging and Detection of Cytolytic Plaques

Section
Modern Virology Assays
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Modern Virology Assays
Sub Section
Antibody Neutralization
Antibody Binding Inhibition
Antibody Neutralization
Cytopathic Effects
Viral Titre
Topic
Automated Imaging and Detection of Cytolytic Plaques
Automated Focus Reduction Neutralization Test Using GFP-expressing RSV
Automated Imaging and Detection of Cytolytic Plaques
Cytomegalovirus Neutralization Assay Using Fluorescence
Zika Virus Microneutralization Assay
Cytomegalovirus Neutralization Assay Using Brightfield
  1. A monolayer of host cells are seeded into a 6-, 12-, or 24-well plate
  2. Mixtures of the virus and various dilutions of the antibodies are produced and allowed to incubate for 2 hours
  3. After incubation, the different mixtures are then added to the host cells and incubated for another 2 hours
  4. The results showed a dose dependent decrease in the formation of cytolytic plaques as the antibody concentration increased
  5. In addition, plaque number and size information can be directly exported into Microsoft Excel for additional plotting and analysis
img-automated-imaging-and-detection-of-cytolytic-plaques-1.PNG

The Celigo™ image cytometer can directly count
cytolytic plaques in plate

img-automated-imaging-and-detection-of-cytolytic-plaques-2.PNG

Plate-view of counted cytolytic plaques treated with different antibody concentrations in a 12-well plate

Plaque count

67

19

4

2

63

13

2

0

Plaque size (µm2)

453017.801

478668.211

375579.65

58492.7559

481462.961

313941.745

382699.547

NaN

Plaque counts and size measurement from the Celigo


For research use only. Not for use in diagnostic procedures.

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