Cytokine Release Syndrome (CRS) can lead to significant tissue damage, and it is an active field of immunology research. We outline a workflow for tissue lysate preparation and quantification of 13 key biomarkers relevant to CRS using the LEGENDplex™ panels and the Omni Bead Ruptor Elite homogenizer. The results show that bead mill homogenization is effective in disrupting tissues for cytokine quantification to support research on inflammatory processes.

The bead mill was able to perform multi-sample, rapid homogenization of spleen and liver tissue in just 20 seconds to create suitable homogenates for subsequent extraction and cytokine quantification. Results are shared for the detection levels of IFN-γ, IL-10, CCL4, IFN-α, CXCL9, CXCL10, TNF- α, IL-6, VEGF, IL-4, CCL3, CCL2, and GM-CSF in an LPS-induced inflammation model (via intraperitoneal injections) and assessed for more local inflammation in abdominal organs of spleen and liver. TNF-α and CXCL10 were notably much higher in LPS-treated groups than PBS-controls.

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For research use only. Not for use in diagnostic procedures.