Skip to main content
Menu
US

Polylysine YOX SPA Imaging Beads, 2 g

Poly-L-lysine SPA beads enhance the binding of negatively-charged membranes to beads.

For research use only. Not for use in diagnostic procedures

Part number: RPQ0328
List price: USD 2,334.00
Your price:
USD 2,334.00
USD 2,334.00 /each

Overview

Poly-L-lysine yttrium oxide (YOx) SPA beads enhance the binding of negatively-charged membranes to beads.

SPA Imaging beads are microspheres containing scintillant which emit light in the red region of the visible spectrum. As a result, these beads are ideally suited to use with a CCD imager.

Two types of core SPA Imaging bead are available - yttrium oxide (YOx) and Polystyrene (PS). A number of biological coatings also exist for each core bead type to enable receptor binding, enzyme, molecular interaction and radioimmunoassays to be investigated.

Scintillation proximity assay (SPA) is a homogeneous and versatile technology for the rapid and sensitive assay of a wide range of biological processes, including applications using enzyme and receptor targets, radioimmunoassays, and molecular interactions. When 3H, 14C, 33P, and 125I radioisotopes decay, they release β-particles (or Auger electrons, in the case of 125I). The distance these particles travel through an aqueous solution is dependent on the energy of the particle. If a radioactive molecule is held in close enough proximity to a SPA Scintillation Bead or a SPA Imaging Bead, the decay particles stimulate the scintillant within the bead to emit light, which is then detected in a PMT-based scintillation counter or on a CCD-based imager, respectively. However, if the radioactive molecule does not associate with the SPA bead, the decay particles will not have sufficient energy to reach the bead and no light will be emitted. This discrimination of binding by proximity means that no physical separation of bound and free radiochemical is required.

Specifications

Application
Drug Discovery & Development
Automation Compatible
No
Bead Type or Material
Yttrium Oxide (YOx)
Brand
SPA Imaging Beads
Coating Treatment
Poly-L-lysine
Poly-lysine
Detection Method
Radiometric
One Unit Contains
2.0 mg
Shipping Conditions
Shipped Ambient
Unit Size
2 g

Resources

1-1 of 1 Resources
Guide Icon
Guide
Optimization of SPA receptor binding assays

Scintillation proximity assay has been successfully applied to receptor binding assays

Scroll Icon