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AlphaLISA High Performance Biotin-Free Human IL-2 Detection Kit, 500 Assay Points

The AlphaLISA® High Performance (HP) Biotin-Free Human IL-2 Detection Kit is designed for the quantitative determination of human IL-2 in serum, buffered solution, or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.

This kit is the new and improved version of part number AL333,biotin-free interleukin-2 detection kit.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Part Number: AL3156HV
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Part Number: AL3156C
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Part Number: AL3156F
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  • Interleukin 2 (IL-2), formerly known as T-cell growth factor (TCGF), is a 15 kDa immunoregulatory lymphokine produced by lectin- or antigen-activated T cells and capable of inducing the thymic expansion of recently activated antigen-specific T lymphocytes. However, since T-cell immunity can be elicited to various agents in the absence of IL-2 in vivo, it is currently thought that the main non-redundant activity of this cytokine is the induction of the suppressor function of CD4+CD25+ regulatory T cells (Treg) in peripheral lymph nodes to ensure suppression of autoreactive T cells that escape negative selection. Moreover, studies suggest that IL-2-mediated regulation of Treg cells is important in the prevention of type 1 diabetes and autoimmune disease.

    Formats
    • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
    • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
    • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
    Features
    • No-wash steps, no separation steps
    • ELISA alternative technology
    • Sensitive detection
    • Broad sample compatibility
    • Small sample volume
    • Results in less than 3 hours
    • Half the time of an ELISA assay

    AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

  • Application
    Protein Analysis & Detection
    Protein Detection
    Assay Points
    500
    Assay Target Class
    Cytokine
    Assay Technology
    Alpha
    Automation Compatible
    Yes
    Brand
    AlphaLISA
    Detection Method
    Alpha
    Experimental Type
    In vitro
    Target Species
    Human
    Therapeutic Area
    Autoimmunity
    Immuno-oncology
    Neuroscience
    Oncology