pHSense eu probes time-resolved fluorescence plate based applications for monitoring internalization of overexpressed and endogenous GPCRs

Understanding GPCR internalization is vital for decoding receptor signaling, trafficking, and drug responses. The pHSense™ Eu platform offers a no-wash, time-resolved fluorescence (TRF) solution for monitoring GPCR internalization in live cells, under both overexpressed and endogenous conditions.

This technical note shows how pH-sensitive Europium-labeled probes respond dynamically to acidic intracellular environments, providing bright, low-background signals ideal for quantifying internalization in real time. Compatible with multiple labeling strategies, including Anti-FLAG, SNAP-tag, and Fab anti-IgG formats, pHSense reagents allow for flexible and high-throughput assay design across a variety of receptor systems.

Key highlights include:

• Monitoring of both constitutive and agonist-induced GPCR internalization
• Case studies in GLP1R internalization using overexpressing and native cell models
• Buffer compatibility across media and physiological conditions
• Optimization guidance for temperature, incubation times, and assay setup

Discover how pHSense probes deliver reliable, high-sensitivity data for GPCR research, empowering drug discovery and functional receptor studies with minimal assay complexity.

For research use only. Not for use in diagnostic procedures.