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Technical Note

Increasing high-content imaging throughput with cell-line multiplexing

Our technical note describes the multiplexing of 4 different cell lines in a single well using Semarion’s SemaCyte® optically-barcoded microcarriers, the Opera Phenix™ Plus high-content screening system and Harmony™ image analysis software. 

Using the cell painting assay and phenotypic profiling, we show that microcarriers from multiplexed wells cluster with the correct cell lines, confirming successful multiplexing. 

Key benefits:

  • 4x throughput increase - analyze multiple cell lines simultaneously per well
  • Reduced reagent costs - fewer wells needed
  • Automated workflow - dedicated Harmony software building block streamlines detection
  • Verified performance - correct clustering by cell type.


For Research Use Only. Not for use in diagnostic procedures.

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Increasing high-content imaging throughput with cell-line multiplexing

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