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Guide

LANCE protein:protein interaction quick start guide

This guide provides researchers with a comprehensive framework for developing and optimizing LANCE TR-FRET assays to study protein-protein interactions, particularly in Revvity Proprietary Information drug discovery contexts. It combines technical depth with practical protocols to support assay design, execution, and analysis.

Key offerings:

  • Detailed explanation of TR-FRET principles and reagent configurations
  • Step-by-step assay development using tagged proteins and toolbox reagents
  • Optimization strategies for incubation times, reagent concentrations, and buffer conditions
  • Guidance on validating assay specificity with small molecule inhibitors like Nutlin-3

For research use only. Not for use in diagnostic procedures.

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LANCE protein:protein interaction quick start guide

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