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Application Note

JAK STAT signaling dynamics in PBMCs and other cell models using AlphaLISA SureFire Ultra

Explore cytokine-driven JAK/STAT activation using AlphaLISA SureFire Ultra assays.

Gain deeper insights into JAK/STAT pathway activation across primary immune cells and immortalized models using a highly sensitive, no-wash immunoassay platform. This application note demonstrates how AlphaLISA SureFire Ultra enables robust and sensitive detection of key JAK and STAT phosphorylation events in physiologically relevant immune systems.

In this application note, you will learn how to:

  • Detect total and phosphorylated JAK and STAT proteins using AlphaLISA SureFire Ultra
  • Monitor cytokine-induced JAK/STAT activation in immune-relevant models
  • Capture pathway dynamics with high sensitivity, even with limited cell numbers
  • Analyze dose-dependent cytokine responses with strong dynamic range
  • Measure JAK1/2 inhibition using a clinically relevant JAK inhibitor (Ruxolitinib)

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JAK STAT signaling dynamics in PBMCs and other cell models using AlphaLISA SureFire Ultra

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