DOPlify WGA V2 Kit for Single-Cell and Low-Input DNA

Fast, low-touch workflow and contamination control

A short protocol delivers microgram-level yield from single cells or other low-input DNA in under 3 hours. Ready-mix reagents, a gentle enzyme-based lysis, and only two tube openings limit hands-on steps and reduce contamination risk. No-template controls show no amplification, supporting confident downstream analysis and QC.

Figure 1: Single-tube workflows for the DOPlify WGA V2 kit. Left: PGT-A WGA (lysis, 10 min incubation, PCR master mix, 2 h amplification). Right: PGT-A + PGT-M with Target Sequence Enrichment (1 h amplification, add specific primers designed by the user, 1 h amplification).

Chemistry, genome representation, and GC performance

DOPlify WGA V2 kit uses advanced DOP-PCR with degenerate primers and sequencing-grade, high-processivity polymerases to initiate representative amplification across the genome from as little as ~6 pg of DNA per cell. Post-WGA libraries show GC content profiles that match unamplified genomic DNA, indicating even amplification and high base quality across GC ranges. This uniformity helps preserve copy-number signals for low-pass sequencing and downstream NGS workflows.

Figure 2: GC bias of libraries prepared with the DOPlify WGA V2 and NEXTFLEX™ Rapid XP DNA-Seq Kits. Normalized coverage stays near 1.0 across the typical GC range with stable mean base quality.

Mitochondrial genome coverage

The workflow preserves mtDNA and enables complete mitochondrial genome coverage. In comparative testing, DOPlify-amplified samples showed improved mtDNA coverage and mutation detection versus a leading single-cell WGA kit.

Yield, applications, and study support

From a single cell, the kit yields sufficient DNA for NGS library prep, copy-number analysis, and other downstream assays. Peer-reviewed studies cited on our product page demonstrate suitability of DOPlify-based WGA for single-cell CNV detection and STR profiling, alongside other methods, supporting use across identification and genetic analysis workflows.

Scalable automation on JANUS™ G3 workstation

Whole-genome amplification and downstream library preparation can be fully automated on the JANUS G3 liquid handler using pre-programmed methods, improving throughput, traceability, and reproducibility for roughly 48 – 96 samples per run, with higher-throughput options available.

• Deleye L, Tilleman L, Vander Plaetsen A-S, Cornelis S, Deforce D, Van Nieuwerburgh F. Performance of four modern whole genome amplification methods for copy number variant detection in single cells. Scientific Reports. 2017;7:3422. doi:10.1038/s41598-017-03711-y.
• Mai AD, Harton GL, Quang VN, et al. Development and clinical application of a preimplantation genetic testing for monogenic disease (PGT-M) for beta thalassemia in Vietnam. Journal of Assisted Reproduction and Genetics. 2020;37(12):3021-3031. doi:10.1007/s10815-020-02006-y.