The purification of small RNA species such as microRNA (miRNA) is challenging because of the low concentrations, small fragment sizes (19-22 nucleotides) and tendency to be found in protein complexes. Due to its essential role in homeostasis, miRNA is an important biomarker for cancer and metabolic disease research.
With only minimal hands-on time, the chemagic miRNA kits enable high throughput and efficient extraction of circulating cell-free small RNA from 200 µl or 1 ml inputs in 24-well or 96-well format. The chemagic miRNA kits obtained equivalent yields to manual methods, while analysis by quantitative PCR and Next Generation Sequencing revealed high diversity and reliable performance.
| Feature | Specification |
|---|---|
| Instrument Compatibility | Manual |
| Nucleic Acid Type | miRNA |
| Sample Type |
Plasma RNA Solutions Serum Urine |
The purification of small RNA species such as microRNA (miRNA) is challenging because of the low concentrations, small fragment sizes (19-22 nucleotides) and tendency to be found in protein complexes. Due to its essential role in homeostasis, miRNA is an important biomarker for cancer and metabolic disease research.
With only minimal hands-on time, the chemagic miRNA kits enable high throughput and efficient extraction of circulating cell-free small RNA from 200 µl or 1 ml inputs in 24-well or 96-well format. The chemagic miRNA kits obtained equivalent yields to manual methods, while analysis by quantitative PCR and Next Generation Sequencing revealed high diversity and reliable performance.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
The images shown are for illustrative purposes only and do not represent the exact contents or configuration of the actual kit. This kit may contain hazardous materials requiring special handling procedures; please refer to the provided Safety Data Sheets and product documentation for complete information.
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The chemagic miRNA Kit is a specialized magnetic bead-based extraction solution designed for the efficient isolation and purification of high-quality microRNA (miRNA) from plasma, urine, or enzymatic reactions. This manual extraction kit leverages Revvity's proven chemagic magnetic bead technology to deliver consistent, reliable results with high purity enabling optimal performance in downstream applications such as quantitative RT-PCR, next-generation sequencing, and microarray analysis. With similar chemistries to chemagic automated miRNA kits, this manual kit offers a scalable solution for research areas including oncology biomarker discovery, liquid biopsy development, cardiovascular disease investigation, neurological disorder studies, infectious disease, and extracellular vesicle characterization.
| Application |
DNA/RNA Isolation
Molecular Biology
Next Generation Sequencing
NGS
Research
RNA
|
|---|---|
| Brand |
chemagic
|
| Format |
Manual
|
| Instrument Compatibility |
Manual
|
| Nucleic Acid Type |
miRNA
|
| Regulatory Status |
RUO
|
| Research Areas |
Biobanking
Genomic Analysis
Oncology
|
| Sample Type |
Plasma
RNA Solutions
Serum
Urine
|
| Sample Volume |
Up to 200 µl
|
| Shelf Life |
24 months
|
| Storage Conditions |
Room Temperature
|
| Technology |
M-PVA Magnetic Bead Technology
|
| Throughput |
Device dependent
|
| Unit Size |
50 preps/kit
|
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