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Application Note

Rapid assessment of cell count and intactness before and after bead mill homogenization of neuroprogenitor cells using a cell counter

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The success of any cell-based assay relies on the fact that proper cell sample preparation was included in the experimental planning. Specifically, downstream molecular analysis of intracellular analytes such as DNA/RNA, soluble proteins, and small molecules depends on an effective and thorough cellular lysis procedure. Traditional cellular lysis methods can be time-consuming and reliant on reagents such as enzymes or detergents to facilitate lysis Therefore, these methods may require additional care to ensure biologically-relevant detection of both genomic and protein expression profiles. In contrast, bead mill homogenization employs mechanical lysis, which could also provide additional time-savings compared to most standard enzymatic lysis methods. In this application note, sample homogenization and cell disruption were performed on neuroprogenitor cells using the Omni Bead Ruptor Elite bead mill homogenizer.  Cell count and viability before and after sample homogenization was assessed using AO/PI dual-staining read on the Cellometer® K2 fluorescent cell counter. 

For research use only. Not for use in diagnostic procedures.

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Rapid assessment of cell count and intactness before and after bead mill homogenization of neuroprogenitor cells using a cell counter

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