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Application Note

Quantification of proteins by UV-Vis absorbance using VICTOR Nivo with microvolume plates

uv-vis-absorbance

Knowing the protein concentration is commonly required in studies of protein biochemistry and molecular biology. A direct method of measuring protein concentration is to determine the absorbance spectrum at 280 nm. This UV-based approach helps scientists rapidly determine the concentration of protein with a procedure that is simple and non-destructive, does not need special reagents or standard curves, and consumes very little sample.

In this study, learn why the VICTOR Nivo multimode plate reader is a very suitable instrument for UV/Vis analysis of proteins with a µDrop Plate. This combination can provide robust and fast read-out of a single sample or up to 16 reads at once.

For research use only. Not for use in diagnostic procedures.

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Quantification of proteins by UV-Vis absorbance using VICTOR Nivo with microvolume plates

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