Pre-clinical animal tissue homogenization poster Omni Bead Ruptor Elite

Disease modelling and the evaluation of toxicity and safety of potential therapies involve the use of common preclinical animals, including mice. As the field continues to progress in fine-tuning the responsible use of animal models together with adoption of technologies such as OOCs or 3D models, a standardized sample prep process is valuable in multiomic profiling of animal tissues for pre-clinical stages of research, drug discovery, and drug development to help the universal goal of lowering the attrition rate of drug candidates. We evaluated the Omni Bead Ruptor Elite bead mill homogenizer for mechanical disruption as a unifying platform for upstream sample homogenization from various relevant tissues for further downstream processes, including protein analysis via immunoassays and genomic applications such as qPCR.

Studies in review within this poster include the following:

• Study 1: High-throughput RNA extraction from mouse kidney, liver, heart, and lung using bead milling vs manual mortar & pestle
• Study 2: Automation-enabled DNA extraction from mouse liver
• Study 3: Homogenization and protein extraction to assess kidney toxicity in mice treated with antimetabolic agents

For research use only. Not for use in diagnostic procedures.