These two notes provide examples and data showing how you can use HTRF assays to study protein-protein interactions of widely varying sizes and affinities.
Partners could get as small as 3 kDa or as big as 150 kDa, and Kd could range from the picoMolar to the microMolar, top-notch results are still at hand!
Get these to see how to perform such assays and interpret data to advance your research
For research use only. Not for use in diagnostic procedures.