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Application Note

Analysis of mitochondrial dynamics in human iPSC-derived neurons using the Operetta CLS High-Content Analysis System

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Mitochondrial dynamics are essential for energy conversion and neuron survival. Understanding changes in mitochondrial dynamics is therefore crucial in developing mitochondria-based therapy options for complex pathological conditions such as cancer, neurological disorders, and metabolic syndromes. Fast kinetic live-cell imaging combined with high-content screening represents a promising strategy to quantitatively track these changes in real time and at large scale.

This application note demonstrates how to investigate mitochondrial dynamics in human iPSC-derived neurons using the Operetta® CLS high-content analysis system.

  • Fast frame rate imaging to accurately capture rapid cellular responses
  • Reliable quantification of mitochondrial dynamics in neurons
  • Flexible kinetic acquisition settings for each channel to avoid unnecessary data acquisition

For research use only, not for use in diagnostic procedures.

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Analysis of mitochondrial dynamics in human iPSC-derived neurons using the Operetta CLS High-Content Analysis System

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