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AlphaLISA Technology Basics

Alpha is a bead-based, no-wash luminescent technology. When Alpha Donor and Acceptor beads are brought together through binding to target, a cascade of chemical reactions is set in motion, creating an amplified signal. These versatile beads are conjugated with various biomolecules that enable detection of unique biological events, offering maximum flexibility. Alpha technology is compatible with a wide range of sample types ranging from simple buffers, cell supernatants, and cell lysates to complex tissue, serum, plasma, and other biological samples.

Alpha offers benefits including:

  • Faster time to results
  • Ability to quantitate large protein complexes, nucleosomes, and viral particles
  • Option for multiplexing
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