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Technical Note

AlphaLISA SureFire Ultra: A highly sensitive and unique no-wash platform for studying TIE2 and eNOS activation in endothelial cells

Discover how AlphaLISA SureFire® Ultra enables robust, quantitative analysis of TIE2–eNOS pathway activation and downstream signaling with high sensitivity, even at low cell inputs.

In this technical note, you will learn how to:

  • Detect total and phosphorylated TIE2 (Tyr992) and eNOS (Ser1177) in endogenous endothelial cells using a homogeneous no wash workflow
  • Characterize dynamic, dose dependent signaling responses to pathway modulators, including pervanadate and the VE PTP inhibitor razuprotafib
  • Quantify upstream and downstream pathway components (AKT and ERK) across a wide dynamic range with minimal sample requirements

For research use only. Not for use in diagnostic procedures.

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AlphaLISA SureFire Ultra: A highly sensitive and unique no-wash platform for studying TIE2 and eNOS activation in endothelial cells

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