Skip to main content
Menu
alsu proteins in whole blood
Application Note

An innovative approach for direct detection of intracellular proteins in human whole blood using AlphaLISA SureFire® Ultra technology.

This application note describes a no-wash approach for detecting total and phosphorylated intracellular proteins in human whole blood using AlphaLISA™ SureFire® Ultra™ immunoassays. The workflow overcomes traditional matrix and handling challenges, enabling sensitive biomarker measurement with minimal sample processing.

In this application note, you will access:

  • A simplified workflow for measuring total and phosphorylated intracellular proteins directly in whole blood
  • Data demonstrating robust analysis of MAPK and JAK/STAT signaling pathways, including PROTAC mediated protein degradatio
  • Practical guidance on selecting the appropriate model (whole blood, PBMCs, or primary macrophages) for low abundance targets such as IRF5
     

To view the full content please answer a few questions

By submitting my personal data, I acknowledge that Revvity Inc. and its affiliates (“Company”) will process my personal data provided above consistent with the Company’s Privacy Policy available here.

CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.

Download Resource

An innovative approach for direct detection of intracellular proteins in human whole blood using AlphaLISA SureFire® Ultra technology.

Download Application Note

Revvity is a trademark of Revvity, Inc. All other trademarks are the property of their respective owners.

Revvity AI Assistant Beta