Working with circulating cell-free DNA (cfDNA) and formalin-fixed paraffin-embedded (FFPE) samples presents a unique set of challenges for molecular biologists, particularly when preparing them for next-generation sequencing (NGS).

The automated workflow of the IDT™ xGen™ cfDNA & FFPE DNA Library Prep v2 MC Kit is vendor-qualified and shows its value when generating libraries for sequencing or preparing samples for hybridization capture. The quality of the automated NGS libraries was measured using key metrics and compared to DNA libraries generated manually. 

Beyond simply preparing the library, the kit allows for generating higher-quality data on the first run, reducing the need for re-sequencing with:

• Unique Molecular Identifiers (UMIs),
• Better representation of the original sample.

Primary applications: 

• Liquid biopsy studies using cfDNA
• Oncology research using FFPE samples
• Minimal residual disease (MRD) research to detect ultra-low-frequency variants
• Whole genome sequencing (WGS) from degraded samples
• Targeted sequencing using hybridization capture
• Identification of germline and somatic variants like single nucleotide polymorphisms (SNPs) and insertions/deletions (indels).

To further increase your lab's efficiency, this method is fully automated and vendor-qualified on the Sciclone™ G3 NGSx liquid handler.

For research use only. Not for use in diagnostic procedures.