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Technical Note

AlphaLISA assay for measurement of core fucosylated glycoproteins using LCA lectin

Measure core fucosylated glycoproteins using a robust, no-wash AlphaLISA™ assay powered by LCA lectin specificity. This technical note outlines assay design, optimization, and validation using representative glycoprotein models.

Homogeneous, no-wash proximity assay using donor and acceptor beads to generate a chemiluminescent signal proportional to fucosylation levels
Demonstrated performance with IgG and lactoferrin, including protocol comparison and antibody titration to refine assay conditions
Proven specificity for core α1,6‑fucosylated glycoproteins, with minimal interference and competitive validation using diverse glycoproteins

For research use only. Not for use in diagnostic procedures.

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AlphaLISA assay for measurement of core fucosylated glycoproteins using LCA lectin

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